2 edition of Growth and morphology of T. cruzi in culture and effect of addition of antiserum. found in the catalog.
Growth and morphology of T. cruzi in culture and effect of addition of antiserum.
M. A. S. Talukder
MSc thesis, Biological Sciences.
This work also provided evidence that intermediate filaments in general might be involved in cell invasion by T. cruzi and tissue homing. In addition, various findings suggest that FLY facilitates in vivo infection by T. cruzi and cooperates with other factors to improve parasite survival to such an extent that it may influence the progression. In agreement with this suggestion, a study of the physiological aspect of T. cruzi during heat-shock showed that at 37° C, when heat-shock was applied, the parasites from the 48 h culture did not display the classical response to the heat treatment, since a general increase in RNA and proteins synthesis was observed (De Carvalho et al., ).
Trypanosoma cruzi (T. cruzi) is a protozoan parasite that causes Chagas disease, a zoonotic disease that can be transmitted to humans by blood-sucking triatomine bugs. T. cruzi is a single-celled eukaryote with a complex life cycle alternating between reduviid bug invertebrate vectors and vertebrate hosts. This article will look at the developmental stages of T. cruzi in the invertebrate. In spite of rather variable results, the authors have demonstrated stage-specific as well as common antigens in amastigotes, epimastigotes and trypomastigotes of Trypanosoma cruzi (strain Y). Antisera to culture or bloodstream [haematozoic] forms were raised in mice and determined by the indirect fluorescent antibody test. Antigens were prepared from parasites grown in vitro in LIT medium.
Changes in morphology and infectivity of cell culture-derived trypomastigotes of Trypanosoma cruzi fibroblast. Mol Biochem Parasitol 6: Requena JM, Jimenez-Ruiz A, Soto M, Assiego R, Santarén JF, Lopez MC, Patarrollo ME, Alonso C Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase. T. cruzi tissue culture forms of Berenice, Tulahuen, Y, and 22 strains were maintained in monolayers of L-6, HeLa, or NIH-3T3 cells grown in Dulbecco's minimal essential medium supplemented with units/ml penicillin, μg/ml streptomycin, and 5% (v/v) fetal calf serum at 37 °C in 5% CO 2.
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Cell proliferation and morphology analysis of the single allele Tcsti1 knockout in T. cruzi. (A) Growth profile of the T. cruzi mutant (gray squares) and wild-type (white triangles) parasites during 9 days of culture in LIT medium. Bars represent the standard Cited by: 3. To establish the effects of glucose on T.
cruzi differentiation, we calculated the percentage of spontaneous metacyclics at various days of the growth curve. The reference point was the standard culture condition (24 mM).
Triatomine parasites differentiated more than those maintained in a prolonged culture (p Cited by: Parasite culture. Epimastigote forms of T.
cruzi were grown in % brain heart infusion medium (BHI), containing hemin and folic acid and supplemented with 10% heat-inactivated fetal bovine serum, at 28°C for 4 days to reach late-log phase growth. For the following experiments, epimastigotes were collected, washed three times in M NaCl, M phosphate-buffer pH (PBS) and Cited by: A study on the effect of tomatine on T.
cruzi growth (Chataing, Concepción, Lobatón, & Usubillaga, ) showed inhibition of in vitro growth after four days of incubation with × 10 -6 M. Cell culture.
Trypanosoma cruzi CL Brener (Zingales et al., ) epimastigotes were grown at 28 °C in RPMI medium (Kendall et al., ).Metacyclic development was induced by addition of 20% Grace’s insect medium (Sullivan, ).Briefly, epimastigotes from a late logarithmic phase culture (− × 10 7 cells ml −1; Cited by: The establishment of T.
cruzi infections in the chicken embryo is possible during the first week of growth, prior to the development of the immune system; an immunological window is open for parasite growth in the epiblast stem cells in the mesodermal, endodermal, neural, and genital crests of the growing embryo.
Chagasin is a Trypanosoma cruzi protein that was recently characterized as a tight-binding inhibitor of papain-like cysteine proteases (CPs). Considering that parasite virulence and morphogenesis depend on the endogenous activity of lysosomal CPs of the cruzipain family, we sought to determine whether chagasin and cruzipain interact in the living cell.
Effect of TcZC3H31 overexpression on T. cruzi growth and differentiation in vitro. (A) Detection of TcZC3HFLAG in epimastigote whole-cell lysates by immunoblot, using an anti-FLAG antibody (M2) and an anti-TcZC3H31 antiserum.
WT, wild-type epimastigotes; FLAG, epimastigotes expressing TcZC3HFLAG. Cells and Culture Media. Epimastigotes of the T. cruzi CL Brener clone were grown in BHT medium as described before (Cazzulo et al., ). Escherichia coli DH5α were used in cloning experiments. Bacteria were grown in Luria-Bertani medium, % NaCl, 1% tryptone (Difco, Detroit, MI), % yeast extract (Difco), and μg/ml ampicillin or The effect of psilostachyin, a natural sesquiterpene lactone, on the growth and viability of cultured epimastigotes of Trypanosoma cruzi (Tulahuen) is reported.
The antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and measuring their viability by using the dye exclusion technique.
The effect on parasite growth was irreversible at concentrations higher than. The inhibition of epimastigote differentiation was also dependent on the time of drug addition to the medium. Proliferation of T. cruzi was also blocked in a dose-dependent manner by the drugs.
The antiserum agglutinates the cells and inhibits their growth in liquid culture medium. Moreover, glucose uptake and glucose-stimulated oxygen uptake of the promastigotes are significantly inhibited by the antiserum.
The results indicate that the antiserum has a profound lethal effect on the in vitro propagation of the parasite. Keywords.
The protozoan parasite Trypanosoma cruzi, the etiological agent of Chagas Disease, undergoes through a complex life cycle where rounds of cell division and differentiation occur initially in the gut of triatominae vectors and, after transmission, inside of infected cells in vertebrate s of the Ras superfamily of GTPases are molecular switches which play pivotal regulatory functions.
Trypanosoma cruzi is the causative agent of Chagas' disease, which affects some 8 - 10 million people in the Americas. The only two drugs approved for the etiological treatment of the disease in. Trypanosoma cruzi, the etiologic Chagas' disease agent, induces changes in protein pattern of the human placenta syncytiotrophoblast.
The glucose transporter protein-1 (GLUT1) is the primary isoform involved in transplacental glucose transport. We carried out in vitro assays to determine if T. cruzi infection would induce changes in placental GLUT1 protein expression under normal and high.
The fluorescence signal was also strongly correlated with the total number of parasites in T. cruzi cultures, providing a simple and rapid means of determining the growth inhibitory dose of anti-T. Trypanosoma cruzi, the etiologic Chagas' disease agent, induces changes in protein pattern of the human placenta glucose transporter protein-1 (GLUT1) is the primary isoform involved in transplacental glucose transport.
We carried out in vitro assays to determine if T. cruzi infection would induce changes in placental GLUT1 protein expression under normal and high.
Trypanosoma cruzi alkaline fraction (FI). Epimastigote forms from the Tulahuén strain of T. cruzi were harvested from cultures in monophasic medium .The epimastigotes were disrupted by seven cycles of freezing and thawing and then centrifuged at supernatant, named F, was subjected to isoelectric focusing (IEF) on agarose gel at pH gradient 3– We ascertain the in vitro Benznidazole (BZN) and Nifurtimox (NFX) susceptibility pattern of epimastigotes, trypomastigotes, and amastigotes of 21 T.
cruzi strains, from patients, reservoir, and triatomine bugs of various geographic origins. Using this panel of isolates, we compute the Epidemiological cut off value (COwt).
Then, the frequency of the susceptible phenotype (Wild type). In addition, the infectivity of T. cruzi clones is modified when it is grown in different hosts; a clone passaged through mice has been shown to be more virulent to mice and guinea pigs than the.
Culture of parasites and host cells. The macrophagotropic Tehuantepec strain of T. cruzi was used in all astigotes were maintained in tissue culture by infection of monolayers of the human glioblastoma cell line 86HG39, grown in RPMI supplemented with 5% fetal calf serum (FCS) ().Parasites released from their dying host cells 5 to 10 days later were collected and centrifuged.The author describes his studies on the effect of specific serum on the morphology and development of Trypanosoma cruzi in culture, for which semi-solid Locke-serum-agar and the CITRI and GROSSOWICZ [this Bulletin,v.
53, ] media were used, while immune sera were produced in rabbits by intravenous injections of cultures. The action of serum upon the trypanosomes was .Moreover, morphological change has not been attributed to heat shock treatment of T.
cruzi culture forms. It was therefore of interest to study HSP90 inhibition in T. cruzi to monitor its effects on HSP synthesis and on the growth and morphological appearance of the parasite and to compare the results with the effects observed with L.